Electrophoretic titration (ET) curves for crude venoms and fractions are performed to determine optimal conditions for separation by ion exchange chromatography, and to evaluate the pI of venom proteins.
A volume of 3.5 µl (5.25-20 µg) sample is separated by electrophoretic titration using a Pharmacia Biotech PhastSystem.
A pH gradient of 3-9 is established on a polyacrylamide isoelectric focusing (IEF 3-9) PhastGel.
The gel is rotated 90˚ and the venom sample applied. The gel is stained with silver nitrate.
.jpg){kind=logo}