Purity determination of fractions using Capillary electrophoresis (CE)
A Beckman Capillary electrophoresis P/ACE 5500 is used to observe the purity of venom fractions.
Ten microliters of 1 mg/mL fractions are mixed with 20 µl of 0.01M Borate buffer, pH 8.3.
The mixture is immediately separated for 10 min at 20 kV, 19.5 µamps, using a 0.01M Borate buffer, pH 8.3 through a 75 µm I.D. X 50 cm (100 x 800 aperture) free zone capillary.
A P/ACE UV absorbance detector at 214 nm is used to detect the peptides.
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